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Messi (37) was not included in the 28-man squad after suffering an ankle injury during last month’s Copa America final in the United States.
The Miami International player is currently undergoing rehabilitation treatment at the American club.
Goalkeeper Franco Armani was absent from the team, as was striker Angel Di Maria, who announced his retirement from international football after winning the Copa America.
Head coach Lionel Scaloni called up midfielder Ezequiel Fernandez and striker Valentin Castellanos for the first time, along with youngsters such as Alejandro Ganacho, Valentin Carbone, Valentin Baco and Matias Süle.
Argentina will face rivals Chile at the Monumental Stadium on September 5 before facing Colombia in Barranquilla five days later.
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